Biology Article Review
Answer the following questions from the attach article. 1.What is the main point or purpose of this paper? 2.What research question or hypothesis is being tested? 3.Identify and describe a pivotal experiment from the paper and what was learned in that specific experiment? (make sure describe each experiment specifically) 4.Identify a weakness in the paper and design a control or series of control experiments that could improve that part of paper? (specifically) 5.If this was your research, what experiment (with control) would you do next?
Miller, A. E., Beilharz, H. T., Malkus, N. P., Lee, S. C. M., Hamamoto, S., Orci, L., Schekman,
R. (2003). Multiple Cargo Binding Sites on the COPII Subunit Sec24p Ensure Capture of Diverse Membrane Proteins into Transport Vesicles. Cell, Vol. 114, 497–509,
Purpose of this paper
The purpose of the study that was carried out by Miller et al. (2003) was to find out whether multiple cargo binding sites on the COPII subunit sec24p guarantees capturing of varied membrane proteins into transport vesicles.
Research question or hypothesis is being tested
From an analysis of the study that was carried out by Miller et al. (2003), it can be explained that the hypothesis that was been tested by the scholars was: Sec22p proteins have multiple independent cargo binding domains that permit for acknowledgment of a varied set of sorting signals. Precisely the hypothesis was:
- It is the direct interaction between a Sec34p subunit and a sorting motif that drives cargo recruitment into COPII vesicles.
A pivotal experiment from the paper and what was learned in that specific experiment
One of the pivotal experiment from the paper involved examining the capability of the vesicles that were generated with mutant proteins to fuse with the Golgi apparatus through incubation of vesicles with acceptor membranes and cytosol succeeded by immunoprecipitation of a mannose moiety.
From that experiment, the hypothesis that claimed there is a direct relation between cargo binding by Sec24p and the conforming presence or absence of that cargo molecule in COPII vesicles was found to be valid.
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Weakness in the paper and how it could be improved
From the review of the research method that was used in the study, it can be explained that the researchers used the ideal research approaches. Nevertheless, it can also be highlighted that the research also had some weaknesses. Precisely, one of the weaknesses of the study can be said to be the fact that only a select types of strains were used in the study thus implying that there is likelihood that the findings might be different if different or more number of strains were used.
Experiment to do next
From the research carried out by Miller et al. (2002), it was found that multiple cargo binding sites on the COPII Subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles. From those findings, the research that can be carried out next as a follow up to the study could involve using types of strains in order to find whether the findings would be similar to the study that has already been carried out by used other types of strains.